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KMID : 1134820120410010041
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Journal of the Korean Society of Food Science and Nutrition
2012 Volume.41 No. 1 p.41 ~ p.48
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Antioxidant Activity and Safety Evaluation of Juice Containing Protaetia brevitarsis
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Park Jae-Hee
Kim So-Yun
Kang Min-Gu
Yoon Min-Soo
Lee Yang-Il
Park Eun-Ju
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Abstract
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The purpose of this study was to assess the antioxidant activity of vegetable extracts (pumpkin, aloe, and artichoke) containing Protaetia brevitarsis (PB) and the clinical and pathological changes in ICR mice after a single oral administration. The total polyphenol (TP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, total radical trapping antioxidant potential (TRAP), oxygen radical absorbance activity (ORAC), and single cell gel electrophoresis assay were done to measure their antioxidant activities. The effect of vegetable extracts containing PB in TP and the ORAC value was significantly higher than those without PB. In addition, all extracts had effective DPPH· scavenging and ABTS·+ scavenging activities. The protective effect of vegetable extracts with/without PB on H2O2-induced DNA damage was found. In a single-dose toxicity study, mortality, body weight, physiological signs, and biochemical analysis were analyzed. Seventy mice were randomly assigned to 7 experimental groups and were administered three vegetable extracts with and without PB (2 g/kg). A full 14 days after administration, no mice mortality was observed in any group. Body weight, physiological signs, and biochemical analysis were never significantly different from those of the control group. Taken together, these findings indicate that vegetable extracts containing PB with antioxidant activities and safety could be applied as medicinal and edible resources in an industrial area.
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KEYWORD
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Protaetia brevitarsis, antioxidant, total phenol, ICR mouse, acute oral toxicity
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